| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities
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| Autores: |
Balti, Rafik
Le Balc'H, Romain
Brodu, Nicolas
Gilbert, Marthe
Le Gouic, Benjamin
Le Gall, Sophie
Sinquin, Corinne
Masse, Anthony
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| Data: |
2018-11
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| Ano: |
2018
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| Palavras-chave: |
Membrane filtration
Exopolysaccharides
Microalga
Concentration
Diafiltration
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| Resumo: |
Exopolysaccharides (EPS) from cell-free Porphyridium cruentum media were concentrated then purified (diafiltration) on a 0.14 µm ceramic membrane. The influence of cross-flow velocities on filtration performances was investigated. Mean permeate fluxes equal to 49.8, 68.9 and 81.9 L.h-1. m-2 were obtained during the concentration at 4 bar for respectively cross-flow velocities inside the membrane lumen equal to 2.5, 3.3 and 4.2 m.s-1; 49.7 L.h-1. m-2 for the diafiltration at 3.3 m.s-1. Permeate fluxes were correctly predicted from polysaccharide concentrations (10 % deviation). Volume reduction factors higher than 7.8 were reached. Rejection rates of polysaccharides and proteins varied according to the cross-flow velocities. Thus, the EPS recovery rate or time of filtration could be modulated following the cross-flow velocity. Polysaccharides were concentrated 6.3 to 10.4 times in such a way that the final sugars concentration reached 1.74 to 2.26 g.L-1. Rheological behavior of filtered solutions changed following the concentration progress. More than 80 % (w/w) of polysaccharides were recovered while 49 % and 99 % of proteins and salts were removed respectively. The filtrations allowed reaching a final monosaccharide content of dry matter equal to 48.9 % against 0.6 % (w/w) initially.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00445/55694/57360.pdf
DOI:10.1016/j.procbio.2018.06.021
https://archimer.ifremer.fr/doc/00445/55694/
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| Editor: |
Elsevier Sci Ltd
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| Formato: |
application/pdf
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| Fonte: |
Process Biochemistry (1359-5113) (Elsevier Sci Ltd), 2018-11 , Vol. 74 , P. 175-184
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| Direitos: |
2018 Elsevier Ltd. All rights reserved.
info:eu-repo/semantics/openAccess
restricted use
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